Stefan del Rey Schnitzler | Periodontist

Periodontics and periodontal disease

Periodontal disease images

Molecular diagnosis in periodontics

Cosmetic Dentistry cost

• ZOOM whitening cost
• Tooth implants cost
• Laser teeth whitening cost

All target sequences can be amplified and visualized

If too little amount of DNA exists, method of detection cannot be sensitive enough. Because of that, a technique called PCR or polymerase chain reaction is used. It consists of obtaining multiple copies from a DNA template. This is accomplished by an enzyme named Taq-polymerase. This enzyme requires two oligonucleotides complementary of the flanking regions of the sequence of interest to yield acceptable specificity. This DNA molecules are designated as primers.

Amplified DNA of desired length can be visualized by using specific DNA dyes called ethidium bromide or, alternatively, SYBR Green.

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